Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
IGF-1 is a growth factor that is closely related to insulin. It carries the same amount of amino acids as insulin and as stated above, it is responsible for the anabolic reactions to GH. When IGF-1 is released by the liver, it then binds to IGF growth factors inside the cells, and causes the stimulation of cell growth. It has many positive effects as it increases nitrogen retention, protein synthesis, and it promotes the growth of new muscle cells, when enough protein is present. The addition of muscle cells is a benefit that IGF-1 brings to the table that anabolic steroids don’t. Anabolic steroids help to increase muscle cell growth, but play no part in cell duplication. This is where anabolic steroids can work very synergistically with IGF-1. While new muscle cells are added from IGF-1, these cells can expect to grow larger from anabolic steroids.